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Microbiology Case Study on Haemophilus influenzae?


You are doing a rotation in the hospital鈥檚 clinical laboratory. A sample of cloudy cerebrospinal fluid (CSF) from a suspected meningitis case arrives and you are told to Gram stain it, and then to plate it on blood agar and chocolate agar. In the Gram stain you find gram-negative rods of varying size and shape. You also find a lot of bacteria inside phagocytic cells. They鈥檙e not diplococci. Colonies grow on both of the plates you inoculated.
Later, the charge nurse tells you that the patient, a 3-year-old girl, has not received any childhood vaccinations.

1. What is the most likely causative organism? Why?
2. Why was the child鈥檚 unvaccinated status helpful in diagnosis?
3. What is causing the cloudiness in the CSF?
4. What other types of infections can this organism cause in children?

pertussis

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